Tandem Repeats Finder Program written by:
Gary Benson
Program in Bioinformatics
Boston University
Version 4.09
Sequence: AWUE01014499.1 Corchorus olitorius cultivar O-4 contig14532, whole genome shotgun sequence
Parameters: 2 7 7 80 10 50 1000
Pmatch=0.80,Pindel=0.10
tuple sizes 0,4,5,7
tuple distances 0, 29, 159, 1000
Length: 45921
ACGTcount: A:0.32, C:0.19, G:0.16, T:0.33
Found at i:37 original size:30 final size:30
Alignment explanation
Indices: 1--383 Score: 540
Period size: 30 Copynumber: 12.7 Consensus size: 30
1 ATGACAACTTCTGGTGTCAATTGCAAGAGC
1 ATGACAACTTCTGGTGTCAATTGCAAGAGC
31 ATGACAACTTCTGGTGTCAATTGCAAGAGC
1 ATGACAACTTCTGGTGTCAATTGCAAGAGC
*
61 ATGACAACTTCTGGTGTCAATTGCAACAGC
1 ATGACAACTTCTGGTGTCAATTGCAAGAGC
*
91 ATGACAACTTCTGGTGTCAATTGCAACAGC
1 ATGACAACTTCTGGTGTCAATTGCAAGAGC
* *
121 ATGACAAGTTCTGGTGTCAATTGCAACAGC
1 ATGACAACTTCTGGTGTCAATTGCAAGAGC
* * *
151 ATGACAATTTCTGGTGTCAACTGCAGGAGC
1 ATGACAACTTCTGGTGTCAATTGCAAGAGC
* *
181 ATGACAATTTCTGGTGTCAATTGCAAGATC
1 ATGACAACTTCTGGTGTCAATTGCAAGAGC
** *
211 ATTGACAACTTCTAATGTCAATTGCAAGACC
1 A-TGACAACTTCTGGTGTCAATTGCAAGAGC
242 ATGACAACTTCTGGTGTCAATTGCAAG-GCC
1 ATGACAACTTCTGGTGTCAATTGCAAGAG-C
*
272 ATGACAACTTCTGGTGTCATTTGCAAG-GCC
1 ATGACAACTTCTGGTGTCAATTGCAAGAG-C
**
302 ATGACAGGTTCTGGTGTCAATTGCAAG-GCC
1 ATGACAACTTCTGGTGTCAATTGCAAGAG-C
*
332 ATGACAACTTCTGGTGTCATTTGCAAG-GCC
1 ATGACAACTTCTGGTGTCAATTGCAAGAG-C
362 ATTGACAACTTCTGGTGTCAAT
1 A-TGACAACTTCTGGTGTCAAT
384 ATATATTAGC
Statistics
Matches: 326, Mismatches: 24, Indels: 5
0.92 0.07 0.01
Matches are distributed among these distances:
30 281 0.86
31 45 0.14
ACGTcount: A:0.28, C:0.21, G:0.22, T:0.28
Consensus pattern (30 bp):
ATGACAACTTCTGGTGTCAATTGCAAGAGC
Found at i:1922 original size:22 final size:22
Alignment explanation
Indices: 1895--1952 Score: 64
Period size: 22 Copynumber: 2.6 Consensus size: 22
1885 AATCACACAG
**
1895 AAATTTTGATAATTTCCCTAAA
1 AAATTTTGATAACCTCCCTAAA
* *
1917 AAATTTT-AGTAACCTCCTTATA
1 AAATTTTGA-TAACCTCCCTAAA
1939 AAATTTTGATAACC
1 AAATTTTGATAACC
1953 ACACTTTGAA
Statistics
Matches: 30, Mismatches: 4, Indels: 4
0.79 0.11 0.11
Matches are distributed among these distances:
21 1 0.03
22 28 0.93
23 1 0.03
ACGTcount: A:0.40, C:0.16, G:0.05, T:0.40
Consensus pattern (22 bp):
AAATTTTGATAACCTCCCTAAA
Found at i:1984 original size:22 final size:22
Alignment explanation
Indices: 1926--1998 Score: 67
Period size: 22 Copynumber: 3.3 Consensus size: 22
1916 AAAATTTTAG
* *
1926 TAACCTC-CTTATAAAATTTTGA
1 TAACCTCAC-TATGAAATTCTGA
* * *
1948 TAACCACACTTTGAAATTGTGA
1 TAACCTCACTATGAAATTCTGA
* *
1970 TAACCTCAGTATGAAATTCTGG
1 TAACCTCACTATGAAATTCTGA
1992 TAACCTC
1 TAACCTC
1999 TTTGATAACC
Statistics
Matches: 41, Mismatches: 9, Indels: 2
0.79 0.17 0.04
Matches are distributed among these distances:
22 40 0.98
23 1 0.02
ACGTcount: A:0.34, C:0.21, G:0.11, T:0.34
Consensus pattern (22 bp):
TAACCTCACTATGAAATTCTGA
Found at i:2057 original size:22 final size:22
Alignment explanation
Indices: 2032--2207 Score: 115
Period size: 22 Copynumber: 7.8 Consensus size: 22
2022 ATAACCAGAT
2032 TATGAAATTTTGATAACCTTCC
1 TATGAAATTTTGATAACCTTCC
* ** * *
2054 TATGCAATTTTTTTAACTTGATCA
1 TATGAAATTTTGATAACCT--TCC
* * *
2078 TATAAAAATTTGGTAACCTTCC
1 TATGAAATTTTGATAACCTTCC
*
2100 TATGAAATTTTGATAACGTCTCCC
1 TATGAAATTTTGATAAC--CTTCC
** *
2124 TACAAAATTTTTATAACC-TCC
1 TATGAAATTTTGATAACCTTCC
* *
2145 ATATGAAATTTTGGTAACCATACC
1 -TATGAAATTTTGATAACC-TTCC
*
2169 -ATGAAATTTGGATAACC-TCC
1 TATGAAATTTTGATAACCTTCC
* *
2189 AAATGAAATTTTGACAACC
1 -TATGAAATTTTGATAACC
2208 ACACTGAAAT
Statistics
Matches: 115, Mismatches: 30, Indels: 18
0.71 0.18 0.11
Matches are distributed among these distances:
20 2 0.02
21 2 0.02
22 76 0.66
24 35 0.30
ACGTcount: A:0.35, C:0.18, G:0.10, T:0.37
Consensus pattern (22 bp):
TATGAAATTTTGATAACCTTCC
Found at i:2208 original size:44 final size:42
Alignment explanation
Indices: 2136--2225 Score: 117
Period size: 44 Copynumber: 2.1 Consensus size: 42
2126 CAAAATTTTT
* ** *
2136 ATAACCTCCATATGAAATTTTGGTAACCATACCATGAAATTTGG
1 ATAACCTCCAAATGAAATTTTGACAACCACA-C-TGAAATTTGG
*
2180 ATAACCTCCAAATGAAATTTTGACAACCACACTGAAATTTTG
1 ATAACCTCCAAATGAAATTTTGACAACCACACTGAAATTTGG
2222 ATAA
1 ATAA
2226 TCACACAAAG
Statistics
Matches: 41, Mismatches: 5, Indels: 2
0.85 0.10 0.04
Matches are distributed among these distances:
42 13 0.32
43 1 0.02
44 27 0.66
ACGTcount: A:0.40, C:0.19, G:0.11, T:0.30
Consensus pattern (42 bp):
ATAACCTCCAAATGAAATTTTGACAACCACACTGAAATTTGG
Found at i:3101 original size:22 final size:20
Alignment explanation
Indices: 3053--3091 Score: 69
Period size: 20 Copynumber: 1.9 Consensus size: 20
3043 ATAAACTCAT
3053 TATGAAATTTCAATAACCTA
1 TATGAAATTTCAATAACCTA
*
3073 TATGAAATTTCATTAACCT
1 TATGAAATTTCAATAACCT
3092 CCCTATGAAA
Statistics
Matches: 18, Mismatches: 1, Indels: 0
0.95 0.05 0.00
Matches are distributed among these distances:
20 18 1.00
ACGTcount: A:0.41, C:0.15, G:0.05, T:0.38
Consensus pattern (20 bp):
TATGAAATTTCAATAACCTA
Found at i:3111 original size:22 final size:22
Alignment explanation
Indices: 3094--3237 Score: 74
Period size: 20 Copynumber: 6.9 Consensus size: 22
3084 ATTAACCTCC
3094 CTATGAAAATTTGATAACCACA
1 CTATGAAAATTTGATAACCACA
*
3116 C--TGAAATTTTGATAACCACA
1 CTATGAAAATTTGATAACCACA
* * * *
3136 TTAT-AAAATCTTGATAATCTCC
1 CTATGAAAAT-TTGATAACCACA
*
3158 CTATG-AAA---GATAATCACA
1 CTATGAAAATTTGATAACCACA
* * **
3176 CTAT-AAAA-TTGGTAGCTGCA
1 CTATGAAAATTTGATAACCACA
* *
3196 CTATGAAAATTTTTATAACCACT
1 CTATGAAAA-TTTGATAACCACA
*
3219 CCATG-AAATTTCGATAACC
1 CTATGAAAATTT-GATAACC
3238 TCCCTATCAG
Statistics
Matches: 89, Mismatches: 22, Indels: 22
0.67 0.17 0.17
Matches are distributed among these distances:
18 15 0.17
20 27 0.30
21 11 0.12
22 26 0.29
23 10 0.11
ACGTcount: A:0.40, C:0.19, G:0.10, T:0.31
Consensus pattern (22 bp):
CTATGAAAATTTGATAACCACA
Found at i:3291 original size:22 final size:22
Alignment explanation
Indices: 3264--3484 Score: 164
Period size: 22 Copynumber: 10.1 Consensus size: 22
3254 ACTGTAATAT
*
3264 CCTCTCTATGTAATTTTGATAA
1 CCTCTCTATGAAATTTTGATAA
* * * *
3286 TCTCTCCATAAAATTTTCATAA
1 CCTCTCTATGAAATTTTGATAA
* *
3308 CCTCCCTATGAAATTTTGTTAA
1 CCTCTCTATGAAATTTTGATAA
* * *
3330 CCTCTCTA-GGAATTTGGTTAA
1 CCTCTCTATGAAATTTTGATAA
*
3351 CCT-TCTTATAAAATTTTGATAA
1 CCTCTC-TATGAAATTTTGATAA
* * *
3373 CCTTTTTATGAAATTTTGGTAA
1 CCTCTCTATGAAATTTTGATAA
* * * *
3395 TCTCTGTATTAAATTTTAATAA
1 CCTCTCTATGAAATTTTGATAA
* *
3417 -CTATACTATGAAGTTTTGATAA
1 CCTCT-CTATGAAATTTTGATAA
*
3439 CCT-TCATATGAAATTTTGGTAA
1 CCTCTC-TATGAAATTTTGATAA
* * *
3461 TCACACTATGAAA-TTTGATAA
1 CCTCTCTATGAAATTTTGATAA
3482 CCT
1 CCT
3485 TCCTAAGTAA
Statistics
Matches: 152, Mismatches: 40, Indels: 15
0.73 0.19 0.07
Matches are distributed among these distances:
20 2 0.01
21 28 0.18
22 118 0.78
23 4 0.03
ACGTcount: A:0.32, C:0.16, G:0.10, T:0.43
Consensus pattern (22 bp):
CCTCTCTATGAAATTTTGATAA
Found at i:3349 original size:21 final size:22
Alignment explanation
Indices: 3305--3353 Score: 64
Period size: 21 Copynumber: 2.3 Consensus size: 22
3295 AAAATTTTCA
*
3305 TAACCTCCCTATGAAATTTTGT
1 TAACCTCCCTATGAAATTTGGT
* *
3327 TAACCTCTCTA-GGAATTTGGT
1 TAACCTCCCTATGAAATTTGGT
3348 TAACCT
1 TAACCT
3354 TCTTATAAAA
Statistics
Matches: 24, Mismatches: 3, Indels: 1
0.86 0.11 0.04
Matches are distributed among these distances:
21 14 0.58
22 10 0.42
ACGTcount: A:0.27, C:0.22, G:0.12, T:0.39
Consensus pattern (22 bp):
TAACCTCCCTATGAAATTTGGT
Found at i:7483 original size:49 final size:47
Alignment explanation
Indices: 7406--7546 Score: 160
Period size: 49 Copynumber: 2.9 Consensus size: 47
7396 CAAGCAATCC
* *
7406 TTTACTTTTCACTGCACTTTTTATCAATTTTTACTACAAAATTGAACT
1 TTTAATTTTCATTGCACTTTTTATCAATTTTTA-TACAAAATTGAACT
* * * *
7454 TTT-ATTTTTACTTGCATCTTTTTCTCAATTTTTAAGACAAAATTGATCT
1 TTTAATTTTCA-TTGCA-CTTTTTATCAATTTTT-ATACAAAATTGAACT
*
7503 TTTAATTTTCATCGCACTTTTTATCAATTTTT-TGACAAAATTGA
1 TTTAATTTTCATTGCACTTTTTATCAATTTTTAT-ACAAAATTGA
7547 TTGGCACGCT
Statistics
Matches: 78, Mismatches: 10, Indels: 11
0.79 0.10 0.11
Matches are distributed among these distances:
47 15 0.19
48 22 0.28
49 34 0.44
50 7 0.09
ACGTcount: A:0.28, C:0.16, G:0.06, T:0.50
Consensus pattern (47 bp):
TTTAATTTTCATTGCACTTTTTATCAATTTTTATACAAAATTGAACT
Found at i:7623 original size:5 final size:5
Alignment explanation
Indices: 7605--7650 Score: 78
Period size: 5 Copynumber: 9.6 Consensus size: 5
7595 GCGCGAACAG
7605 TAAGA T-AG- TAAGA TAAGA TAAGA TAAGA TAAGA TAAGA TAAGA TAA
1 TAAGA TAAGA TAAGA TAAGA TAAGA TAAGA TAAGA TAAGA TAAGA TAA
7651 TAACAAGCAA
Statistics
Matches: 39, Mismatches: 0, Indels: 4
0.91 0.00 0.09
Matches are distributed among these distances:
3 1 0.03
4 4 0.10
5 34 0.87
ACGTcount: A:0.59, C:0.00, G:0.20, T:0.22
Consensus pattern (5 bp):
TAAGA
Found at i:15636 original size:46 final size:45
Alignment explanation
Indices: 15584--15679 Score: 115
Period size: 46 Copynumber: 2.1 Consensus size: 45
15574 CATGAGAGCA
*
15584 CTCAAATTGAT-ATCATACCATTTGGA-GTAATGTTGAATGAATGGAG
1 CTCAAATTGATGAT-ATACCATGTGGATG-AATGTTGAATGAA-GGAG
* * *
15630 CTCAAATTGATGATATGCTATGTGGATGAATGTTGATTGAAGGAG
1 CTCAAATTGATGATATACCATGTGGATGAATGTTGAATGAAGGAG
15675 CTCAA
1 CTCAA
15680 TGTGATAACG
Statistics
Matches: 44, Mismatches: 4, Indels: 5
0.83 0.08 0.09
Matches are distributed among these distances:
45 9 0.20
46 32 0.73
47 3 0.07
ACGTcount: A:0.33, C:0.10, G:0.24, T:0.32
Consensus pattern (45 bp):
CTCAAATTGATGATATACCATGTGGATGAATGTTGAATGAAGGAG
Found at i:15964 original size:22 final size:22
Alignment explanation
Indices: 15939--16017 Score: 70
Period size: 22 Copynumber: 3.5 Consensus size: 22
15929 GGATATGAAC
15939 AAAATTTCATAGAGTGGTTATA
1 AAAATTTCATAGAGTGGTTATA
* * *
15961 AAAAATTCATA-AGGAGGTTATC
1 AAAATTTCATAGA-GTGGTTATA
* * * *
15983 AAAATATCATAGGAATGTTTATT
1 AAAATTTCATA-GAGTGGTTATA
16006 AAAATTTCATAG
1 AAAATTTCATAG
16018 CTAGGTTATC
Statistics
Matches: 44, Mismatches: 10, Indels: 6
0.73 0.17 0.10
Matches are distributed among these distances:
21 1 0.02
22 27 0.61
23 15 0.34
24 1 0.02
ACGTcount: A:0.44, C:0.06, G:0.15, T:0.34
Consensus pattern (22 bp):
AAAATTTCATAGAGTGGTTATA
Found at i:16201 original size:21 final size:21
Alignment explanation
Indices: 16161--16203 Score: 61
Period size: 22 Copynumber: 2.0 Consensus size: 21
16151 CTAAAACTAG
16161 TTATTTAAAATATATTTATTAT
1 TTATTTAAAATATA-TTATTAT
16183 TTATTTAATAATATA-TATTAT
1 TTATTTAA-AATATATTATTAT
16204 ATCTAAGATA
Statistics
Matches: 20, Mismatches: 0, Indels: 3
0.87 0.00 0.13
Matches are distributed among these distances:
21 6 0.30
22 8 0.40
23 6 0.30
ACGTcount: A:0.42, C:0.00, G:0.00, T:0.58
Consensus pattern (21 bp):
TTATTTAAAATATATTATTAT
Found at i:20427 original size:15 final size:15
Alignment explanation
Indices: 20396--20424 Score: 51
Period size: 15 Copynumber: 2.0 Consensus size: 15
20386 AAATTTCAAG
20396 AAAATAAAATATATT
1 AAAATAAAATATATT
20411 AAAATAAAA-ATATT
1 AAAATAAAATATATT
20425 TAATTTTTAT
Statistics
Matches: 14, Mismatches: 0, Indels: 1
0.93 0.00 0.07
Matches are distributed among these distances:
14 5 0.36
15 9 0.64
ACGTcount: A:0.69, C:0.00, G:0.00, T:0.31
Consensus pattern (15 bp):
AAAATAAAATATATT
Found at i:20729 original size:29 final size:28
Alignment explanation
Indices: 20685--20740 Score: 94
Period size: 29 Copynumber: 2.0 Consensus size: 28
20675 TTCTTCAAAC
*
20685 TTTCTAATTTCAAGAACGCTCAAGAACA
1 TTTCTAATTTCAAGAACGCTAAAGAACA
20713 TTTCTAATCTTCAAGAACGCTAAAGAAC
1 TTTCTAAT-TTCAAGAACGCTAAAGAAC
20741 GTGGAATAAC
Statistics
Matches: 26, Mismatches: 1, Indels: 1
0.93 0.04 0.04
Matches are distributed among these distances:
28 8 0.31
29 18 0.69
ACGTcount: A:0.39, C:0.21, G:0.11, T:0.29
Consensus pattern (28 bp):
TTTCTAATTTCAAGAACGCTAAAGAACA
Found at i:40595 original size:25 final size:25
Alignment explanation
Indices: 40558--40638 Score: 93
Period size: 25 Copynumber: 3.5 Consensus size: 25
40548 ATTCACTATT
*
40558 TATTATCTTTTTATTTATTTTCAAC
1 TATTATCTATTTATTTATTTTCAAC
*
40583 TATTATCTATTTATTTA----CTA-
1 TATTATCTATTTATTTATTTTCAAC
*
40603 T-TTATCTTTTTATTTATTTTCAAC
1 TATTATCTATTTATTTATTTTCAAC
40627 TATTATCTATTT
1 TATTATCTATTT
40639 TTTTTACTAT
Statistics
Matches: 45, Mismatches: 5, Indels: 12
0.73 0.08 0.19
Matches are distributed among these distances:
19 14 0.31
20 1 0.02
21 2 0.04
23 2 0.04
24 1 0.02
25 25 0.56
ACGTcount: A:0.25, C:0.11, G:0.00, T:0.64
Consensus pattern (25 bp):
TATTATCTATTTATTTATTTTCAAC
Found at i:40609 original size:19 final size:18
Alignment explanation
Indices: 40581--40618 Score: 58
Period size: 19 Copynumber: 2.1 Consensus size: 18
40571 TTTATTTTCA
40581 ACTATTATCTATTTATTT
1 ACTATTATCTATTTATTT
*
40599 ACTATTTATCTTTTTATTT
1 ACTA-TTATCTATTTATTT
40618 A
1 A
40619 TTTTCAACTA
Statistics
Matches: 18, Mismatches: 1, Indels: 1
0.90 0.05 0.05
Matches are distributed among these distances:
18 4 0.22
19 14 0.78
ACGTcount: A:0.26, C:0.11, G:0.00, T:0.63
Consensus pattern (18 bp):
ACTATTATCTATTTATTT
Found at i:40665 original size:45 final size:44
Alignment explanation
Indices: 40560--40695 Score: 191
Period size: 44 Copynumber: 3.0 Consensus size: 44
40550 TCACTATTTA
40560 TTATCTTTTTATTTATTTTCAACTATTATCTATTTATTTACTAT
1 TTATCTTTTTATTTATTTTCAACTATTATCTATTTATTTACTAT
*
40604 TTATCTTTTTATTTATTTTCAACTATTATCTATTTTTTTTACTAT
1 TTATCTTTTTATTTATTTTCAACTATTATCTA-TTTATTTACTAT
* * * * *
40649 TTATCTTTTTACTTATTAATTTAGCTATTACCTATTTATTTATTAT
1 TTATCTTTTTATTTATT--TTCAACTATTATCTATTTATTTACTAT
40695 T
1 T
40696 ATTATCCTTT
Statistics
Matches: 82, Mismatches: 7, Indels: 4
0.88 0.08 0.04
Matches are distributed among these distances:
44 32 0.39
45 27 0.33
46 11 0.13
47 12 0.15
ACGTcount: A:0.24, C:0.11, G:0.01, T:0.64
Consensus pattern (44 bp):
TTATCTTTTTATTTATTTTCAACTATTATCTATTTATTTACTAT
Found at i:40748 original size:8 final size:8
Alignment explanation
Indices: 40714--40782 Score: 52
Period size: 8 Copynumber: 8.8 Consensus size: 8
40704 TTTTAGCTAC
40714 CTATTTAT
1 CTATTTAT
40722 CTA-TTATT
1 CTATTTA-T
* *
40730 CTCTGTAT
1 CTATTTAT
40738 CTATTTAT
1 CTATTTAT
40746 CTATTTAT
1 CTATTTAT
* * *
40754 CTCTATAG
1 CTATTTAT
40762 CTATTTAT
1 CTATTTAT
* *
40770 TTTTTTAT
1 CTATTTAT
40778 -TATTT
1 CTATTT
40783 TTTTAAACTT
Statistics
Matches: 46, Mismatches: 13, Indels: 5
0.72 0.20 0.08
Matches are distributed among these distances:
7 7 0.15
8 37 0.80
9 2 0.04
ACGTcount: A:0.22, C:0.13, G:0.03, T:0.62
Consensus pattern (8 bp):
CTATTTAT
Found at i:40777 original size:24 final size:24
Alignment explanation
Indices: 40714--40769 Score: 78
Period size: 24 Copynumber: 2.3 Consensus size: 24
40704 TTTTAGCTAC
* *
40714 CTATTTATCTA-TTATTCTCTGTAT
1 CTATTTATCTATTTA-TCTCTATAG
40738 CTATTTATCTATTTATCTCTATAG
1 CTATTTATCTATTTATCTCTATAG
40762 CTATTTAT
1 CTATTTAT
40770 TTTTTTATTA
Statistics
Matches: 29, Mismatches: 2, Indels: 2
0.88 0.06 0.06
Matches are distributed among these distances:
24 26 0.90
25 3 0.10
ACGTcount: A:0.23, C:0.16, G:0.04, T:0.57
Consensus pattern (24 bp):
CTATTTATCTATTTATCTCTATAG
Found at i:45061 original size:49 final size:50
Alignment explanation
Indices: 44931--45074 Score: 184
Period size: 49 Copynumber: 2.9 Consensus size: 50
44921 ACTTTCCCTT
* * *
44931 AATTGAAAAC-TAAAACCTGGTGGGAACTTTCCCAATTTGCAAAAGAGCTA
1 AATTGAATACTTAAAAACTGATGGGAACTTTCCCAATTTG-AAAAGAGCTA
* * * **
44981 GATTGAATACTTTGAAAACTGATGGGAACTTTCCCGATTTGAAAA-ATTTA
1 AATTGAATAC-TTAAAAACTGATGGGAACTTTCCCAATTTGAAAAGAGCTA
45031 AATTGAATACTTAAAAACTGATGGGAACTTTCCCAATTTGAAAA
1 AATTGAATACTTAAAAACTGATGGGAACTTTCCCAATTTGAAAA
45075 CTTAAACCTG
Statistics
Matches: 81, Mismatches: 11, Indels: 5
0.84 0.11 0.05
Matches are distributed among these distances:
49 32 0.40
50 20 0.25
51 4 0.05
52 25 0.31
ACGTcount: A:0.40, C:0.15, G:0.17, T:0.29
Consensus pattern (50 bp):
AATTGAATACTTAAAAACTGATGGGAACTTTCCCAATTTGAAAAGAGCTA
Done.