Tandem Repeats Finder Program written by:
Gary Benson
Program in Bioinformatics
Boston University
Version 4.09
Sequence: AWUE01017021.1 Corchorus olitorius cultivar O-4 contig17054, whole genome shotgun sequence
Parameters: 2 7 7 80 10 50 1000
Pmatch=0.80,Pindel=0.10
tuple sizes 0,4,5,7
tuple distances 0, 29, 159, 1000
Length: 19287
ACGTcount: A:0.31, C:0.20, G:0.17, T:0.33
Found at i:1971 original size:14 final size:15
Alignment explanation
Indices: 1952--1981 Score: 53
Period size: 15 Copynumber: 2.1 Consensus size: 15
1942 CAATCAAAGC
1952 AATAAT-CAAGGAAA
1 AATAATGCAAGGAAA
1966 AATAATGCAAGGAAA
1 AATAATGCAAGGAAA
1981 A
1 A
1982 TTAAAAAGAT
Statistics
Matches: 15, Mismatches: 0, Indels: 1
0.94 0.00 0.06
Matches are distributed among these distances:
14 6 0.40
15 9 0.60
ACGTcount: A:0.63, C:0.07, G:0.17, T:0.13
Consensus pattern (15 bp):
AATAATGCAAGGAAA
Found at i:2360 original size:21 final size:21
Alignment explanation
Indices: 2336--2390 Score: 74
Period size: 21 Copynumber: 2.6 Consensus size: 21
2326 GGCACTGAAT
* *
2336 GGTGATGGCACGGACATAGCC
1 GGTGGTGGCACGGACATAACC
* *
2357 GGTGGTGGCACGGGCTTAACC
1 GGTGGTGGCACGGACATAACC
2378 GGTGGTGGCACGG
1 GGTGGTGGCACGG
2391 TGAATGGTCG
Statistics
Matches: 30, Mismatches: 4, Indels: 0
0.88 0.12 0.00
Matches are distributed among these distances:
21 30 1.00
ACGTcount: A:0.16, C:0.22, G:0.45, T:0.16
Consensus pattern (21 bp):
GGTGGTGGCACGGACATAACC
Found at i:5592 original size:29 final size:29
Alignment explanation
Indices: 5524--5613 Score: 110
Period size: 29 Copynumber: 3.0 Consensus size: 29
5514 GAAAATAAAG
*
5524 AAATTTTTTTTTTTCTAAAAACGCAGGAACA
1 AAATTTTTTTTTTT-TAAAAACGCA-AAACA
*
5555 AGAAATTTTTTTTTTTAGAAAACGCAAAAC-
1 A-AATTTTTTTTTTTTA-AAAACGCAAAACA
5585 AAATTTTTTTTTTTTCAAAAACGCAAAAC
1 AAATTTTTTTTTTTT-AAAAACGCAAAAC
5614 TAATAAATTA
Statistics
Matches: 53, Mismatches: 3, Indels: 8
0.83 0.05 0.12
Matches are distributed among these distances:
29 25 0.47
30 2 0.04
31 6 0.11
32 20 0.38
ACGTcount: A:0.41, C:0.12, G:0.08, T:0.39
Consensus pattern (29 bp):
AAATTTTTTTTTTTTAAAAACGCAAAACA
Found at i:5717 original size:21 final size:21
Alignment explanation
Indices: 5656--5717 Score: 70
Period size: 23 Copynumber: 2.9 Consensus size: 21
5646 TTTATGAAAA
**
5656 ACGCAAAAACCTTTTTTTATG
1 ACGCAAAAAAATTTTTTTATG
*
5677 ACGCAGAAACAAAATTTTTTATG
1 ACGCA-AAA-AAATTTTTTTATG
*
5700 ACGCAAAAATATTTTTTT
1 ACGCAAAAAAATTTTTTT
5718 TTCCGATGCA
Statistics
Matches: 34, Mismatches: 5, Indels: 4
0.79 0.12 0.09
Matches are distributed among these distances:
21 13 0.38
22 6 0.18
23 15 0.44
ACGTcount: A:0.39, C:0.15, G:0.10, T:0.37
Consensus pattern (21 bp):
ACGCAAAAAAATTTTTTTATG
Found at i:5908 original size:29 final size:29
Alignment explanation
Indices: 5867--5934 Score: 109
Period size: 29 Copynumber: 2.3 Consensus size: 29
5857 GTCTATTTTT
5867 CCTTCCCCTGGCAAAAACCAAAAAAAGTTC
1 CCTTCCCC-GGCAAAAACCAAAAAAAGTTC
* *
5897 CCTTCCCCGGCAAATACCAGAAAAAGTTC
1 CCTTCCCCGGCAAAAACCAAAAAAAGTTC
5926 CCTTCCCCG
1 CCTTCCCCG
5935 TCAACGGCGC
Statistics
Matches: 36, Mismatches: 2, Indels: 1
0.92 0.05 0.03
Matches are distributed among these distances:
29 28 0.78
30 8 0.22
ACGTcount: A:0.32, C:0.38, G:0.12, T:0.18
Consensus pattern (29 bp):
CCTTCCCCGGCAAAAACCAAAAAAAGTTC
Found at i:6138 original size:15 final size:16
Alignment explanation
Indices: 6114--6153 Score: 64
Period size: 15 Copynumber: 2.6 Consensus size: 16
6104 AGAGATTGAC
*
6114 AGAAAGCAATTAAA-T
1 AGAAAACAATTAAACT
6129 AGAAAACAATTAAACT
1 AGAAAACAATTAAACT
6145 AGAAAACAA
1 AGAAAACAA
6154 AGCAGAGTAA
Statistics
Matches: 23, Mismatches: 1, Indels: 1
0.92 0.04 0.04
Matches are distributed among these distances:
15 13 0.57
16 10 0.43
ACGTcount: A:0.65, C:0.10, G:0.10, T:0.15
Consensus pattern (16 bp):
AGAAAACAATTAAACT
Found at i:11165 original size:47 final size:49
Alignment explanation
Indices: 11016--12440 Score: 954
Period size: 47 Copynumber: 28.5 Consensus size: 49
11006 TATTACTGCT
* * * * *
11016 TTTTACTTTTTTAGTTTAATTACTCTGAACTAAACTAATTACTCTTACTCTTTC
1 TTTTAC-TATTTAGTTTAATTACCCAGAATTAAACTAATTCCTC-T--TC-TTC
* ** * * *
11070 TTTTACTCTTTAACTTAATTACTCAGAATTAAACTAATTACTGTTTACTCTTTC
1 TTTTACTATTTAGTTTAATTACCCAGAATTAAACTAATT-C-CTCT--TC-TTC
* * *
11124 TTTTACTCTTTAGTTTAATTACCCAGAATTAAACTAATTACTGTTTACTCTTTC
1 TTTTACTATTTAGTTTAATTACCCAGAATTAAACTAATT-C-CTCT--TC-TTC
*
11178 TTTTACTCTTTAGTTTAATTACCCAGAATTAAACTAATTACTGCTTTACTTCTTTC
1 TTTTACTATTTAGTTTAATTACCCAGAATTAAACTAATT-C--C--T-CTTC-TTC
* * * ** * *
11234 TTTTACTCTTTAGTTTAATTACTCATAATTAAACTAATTATTGTT-TAC
1 TTTTACTATTTAGTTTAATTACCCAGAATTAAACTAATTCCTCTTCTTC
* *
11282 TCTTT-CT-TTTACTCTTTTAATTACCCAGAATTAAACTAATTACTGTTTTACTTCTTTC
1 T-TTTACTATTTA---GTTTAATTACCCAGAATTAAACTAATT-C----CT-CTTC-TTC
* *
11340 TTTTACTCTTTAGTTTAATTACCCATAATTAAACTAATTACTGCTTTACTTCTTC
1 TTTTACTATTTAGTTTAATTACCCAGAATTAAACTAATT-C--C--T-CTTCTTC
* * *
11395 TTTTACTCTTTAGTTTAATTGCCCAGAATTAAACTAACTTATGCTTTACTTCTTC
1 TTTTACTATTTAGTTTAATTACCCAGAATTAAACTAA--T-T-C-CT-CTTCTTC
* * * *
11450 CTTTACTCTTTAGTTTAATTGCCCATAATTAAACTAACTTCTGCTTTGCTTCTTC
1 TTTTACTATTTAGTTTAATTACCCAGAATTAAACTAA-TTC--C--T-CTTCTTC
* * * * *
11505 TTTTACTCTTTAGTTTAATTGCCCAGAATTAAACTGA--CTTCTGCTTC
1 TTTTACTATTTAGTTTAATTACCCAGAATTAAACTAATTCCTCTTCTTC
* * *
11552 TTTTACTATTTAGTTTAATTGCCCAGAATTAAACTGA--CTTCTTCTTC
1 TTTTACTATTTAGTTTAATTACCCAGAATTAAACTAATTCCTCTTCTTC
* * *
11599 TTTTACTATTTAGTTTAATTAACCTGAATTAAACTGACTTCTGCTTTACTTCTTC
1 TTTTACTATTTAGTTTAATTACCCAGAATTAAACT-AATTC--C--T-CTTCTTC
* * *
11654 TTTTACTCTTTAGTTTAATTGCCCAGAATTAAACTGACTTCTGCTTTACTTCTTC
1 TTTTACTATTTAGTTTAATTACCCAGAATTAAACT-AATTC--C--T-CTTCTTC
* * *
11709 TTTTACTCTTTAGTTTAATTACCCAAAATTAAACCAA--CCTCTTCTTC
1 TTTTACTATTTAGTTTAATTACCCAGAATTAAACTAATTCCTCTTCTTC
* * * * ** *** * * * **
11756 TTTTACTCTTTAGTTAAACTAACC--TCTTCTTCT-TTTACTATT-TAA
1 TTTTACTATTTAGTTTAATTACCCAGAATTAAACTAATTCCTCTTCTTC
* * * *** *
11801 TTTAAACTAACCTCTT-CTTCT-TTTACTCTTTAGTTAAACTAA--CCTCTTCTTC
1 TTT-TACT-A--T-TTAGTT-TAATTAC-CCAGAATTAAACTAATTCCTCTTCTTC
* * * *
11853 -TTTACTATTTAGTTTAATTACCTAGAATTATACCAA--CCTCTT-TTT
1 TTTTACTATTTAGTTTAATTACCCAGAATTAAACTAATTCCTCTTCTTC
*
11898 TCTTTACTATTTATTTTAATTACCCAGAATTAAACTAA--CCTCTTCTTC
1 T-TTTACTATTTAGTTTAATTACCCAGAATTAAACTAATTCCTCTTCTTC
* * * *
11946 TTTTACTATTTAATTTGATTACCTAGAATTAAACCAA--CCTCTTCTTC
1 TTTTACTATTTAGTTTAATTACCCAGAATTAAACTAATTCCTCTTCTTC
**
11993 -TTTACTATTTAGTTTAATTACCTGGAATTAAACTAA--CCTCTTCTTC
1 TTTTACTATTTAGTTTAATTACCCAGAATTAAACTAATTCCTCTTCTTC
* * *
12039 TTTTACTATTTAGTTTAATCACCTAGAATTAAACTAA--CCTCTCCTTC
1 TTTTACTATTTAGTTTAATTACCCAGAATTAAACTAATTCCTCTTCTTC
* * *
12086 TTTTACTATTTAGTTTAATTACCTAGAATTAAAGT-A-GCCTCTTCTTC
1 TTTTACTATTTAGTTTAATTACCCAGAATTAAACTAATTCCTCTTCTTC
* *
12133 TTTTACTATTTAGTTTAATCACCTAGAATTAAACTAA--CCTCTTCTTC
1 TTTTACTATTTAGTTTAATTACCCAGAATTAAACTAATTCCTCTTCTTC
* * *
12180 TTTTACTATTTAGTTTAATTACCTATAATTAAACT-A-CCCTCTTCTTC
1 TTTTACTATTTAGTTTAATTACCCAGAATTAAACTAATTCCTCTTCTTC
* * *
12227 TTATACTATTTAGTTTAATTACCTAGAATTAAACTAA--CTTCTTCTTC
1 TTTTACTATTTAGTTTAATTACCCAGAATTAAACTAATTCCTCTTCTTC
* * *
12274 TTTTACTATTTAGTTTAATTACCTAGAATTAAACT-A-GCATCTTCTTC
1 TTTTACTATTTAGTTTAATTACCCAGAATTAAACTAATTCCTCTTCTTC
* * *
12321 TTTTACTATTTAGTTTAATTACCTAGAATTAAACTAACCTCTTCTTCTTC
1 TTTTACTATTTAGTTTAATTACCCAGAATTAAACTAA-TTCCTCTTCTTC
* **
12371 TTTTACTATTTAGTTTAATTACCTAGAATTAAACT-A-GGCTCTTCTTC
1 TTTTACTATTTAGTTTAATTACCCAGAATTAAACTAATTCCTCTTCTTC
*
12418 TTTTACTATTTAGTTCAATTACC
1 TTTTACTATTTAGTTTAATTACC
12441 TGATTTAAAC
Statistics
Matches: 1171, Mismatches: 137, Indels: 133
0.81 0.10 0.09
Matches are distributed among these distances:
45 9 0.01
46 71 0.06
47 523 0.45
48 13 0.01
49 10 0.01
50 78 0.07
51 7 0.01
52 8 0.01
53 29 0.02
54 111 0.09
55 217 0.19
56 78 0.07
57 5 0.00
58 8 0.01
59 4 0.00
ACGTcount: A:0.27, C:0.20, G:0.05, T:0.48
Consensus pattern (49 bp):
TTTTACTATTTAGTTTAATTACCCAGAATTAAACTAATTCCTCTTCTTC
Found at i:11425 original size:55 final size:55
Alignment explanation
Indices: 11016--11742 Score: 854
Period size: 55 Copynumber: 13.6 Consensus size: 55
11006 TATTACTGCT
* * * *
11016 TTTTACTTTTTTAGTTTAATTACTCTGAACTAAACTAATTACT-C-TTAC-TCTTTC
1 TTTTAC-TCTTTAGTTTAATTACCCAGAATTAAACTAATTACTGCTTTACTTC-TTC
** *
11070 TTTTACTCTTTAACTTAATTACTCAGAATTAAACTAATTACTG-TTTAC-TCTTTC
1 TTTTACTCTTTAGTTTAATTACCCAGAATTAAACTAATTACTGCTTTACTTC-TTC
11124 TTTTACTCTTTAGTTTAATTACCCAGAATTAAACTAATTACTG-TTTAC-TCTTTC
1 TTTTACTCTTTAGTTTAATTACCCAGAATTAAACTAATTACTGCTTTACTTC-TTC
11178 TTTTACTCTTTAGTTTAATTACCCAGAATTAAACTAATTACTGCTTTACTTCTTTC
1 TTTTACTCTTTAGTTTAATTACCCAGAATTAAACTAATTACTGCTTTACTTC-TTC
* * *
11234 TTTTACTCTTTAGTTTAATTACTCATAATTAAACTAATTATTG-TTTAC-TCTTTC
1 TTTTACTCTTTAGTTTAATTACCCAGAATTAAACTAATTACTGCTTTACTTC-TTC
*
11288 TTTTACTC--T--TTTAATTACCCAGAATTAAACTAATTACTGTTTTACTTCTTTC
1 TTTTACTCTTTAGTTTAATTACCCAGAATTAAACTAATTACTGCTTTACTTC-TTC
*
11340 TTTTACTCTTTAGTTTAATTACCCATAATTAAACTAATTACTGCTTTACTTCTTC
1 TTTTACTCTTTAGTTTAATTACCCAGAATTAAACTAATTACTGCTTTACTTCTTC
*
11395 TTTTACTCTTTAGTTTAATTGCCCAGAATTAAACTAACTTA-TGCTTTACTTCTTC
1 TTTTACTCTTTAGTTTAATTACCCAGAATTAAACTAA-TTACTGCTTTACTTCTTC
* * * *
11450 CTTTACTCTTTAGTTTAATTGCCCATAATTAAACTAACTT-CTGCTTTGCTTCTTC
1 TTTTACTCTTTAGTTTAATTACCCAGAATTAAACTAA-TTACTGCTTTACTTCTTC
* * *
11505 TTTTACTCTTTAGTTTAATTGCCCAGAATTAAAC---TGA---C-TT-CTGCTTC
1 TTTTACTCTTTAGTTTAATTACCCAGAATTAAACTAATTACTGCTTTACTTCTTC
* * *
11552 TTTTACTATTTAGTTTAATTGCCCAGAATTAAAC---TGA---C-TT-CTTCTTC
1 TTTTACTCTTTAGTTTAATTACCCAGAATTAAACTAATTACTGCTTTACTTCTTC
* * * *
11599 TTTTACTATTTAGTTTAATTAACCTGAATTAAACTGACTT-CTGCTTTACTTCTTC
1 TTTTACTCTTTAGTTTAATTACCCAGAATTAAACT-AATTACTGCTTTACTTCTTC
* *
11654 TTTTACTCTTTAGTTTAATTGCCCAGAATTAAACTGACTT-CTGCTTTACTTCTTC
1 TTTTACTCTTTAGTTTAATTACCCAGAATTAAACT-AATTACTGCTTTACTTCTTC
*
11709 TTTTACTCTTTAGTTTAATTACCCAAAATTAAAC
1 TTTTACTCTTTAGTTTAATTACCCAGAATTAAAC
11743 CAACCTCTTC
Statistics
Matches: 614, Mismatches: 37, Indels: 43
0.88 0.05 0.06
Matches are distributed among these distances:
47 82 0.13
48 2 0.00
49 1 0.00
50 27 0.04
51 7 0.01
52 15 0.02
53 32 0.05
54 127 0.21
55 235 0.38
56 86 0.14
ACGTcount: A:0.26, C:0.19, G:0.06, T:0.48
Consensus pattern (55 bp):
TTTTACTCTTTAGTTTAATTACCCAGAATTAAACTAATTACTGCTTTACTTCTTC
Found at i:11811 original size:33 final size:32
Alignment explanation
Indices: 11737--11866 Score: 208
Period size: 32 Copynumber: 4.1 Consensus size: 32
11727 TTACCCAAAA
* *
11737 TTAAACCAACCTCTTCTTCTTTTACTCTTTAG
1 TTAAACTAACCTCTTCTTCTTTTACTATTTAG
*
11769 TTAAACTAACCTCTTCTTCTTTTACTATTTAAT
1 TTAAACTAACCTCTTCTTCTTTTACTATTT-AG
*
11802 TTAAACTAACCTCTTCTTCTTTTACTCTTTAG
1 TTAAACTAACCTCTTCTTCTTTTACTATTTAG
11834 TTAAACTAACCTCTTCTTC-TTTACTATTTAG
1 TTAAACTAACCTCTTCTTCTTTTACTATTTAG
11865 TT
1 TT
11867 TAATTACCTA
Statistics
Matches: 91, Mismatches: 6, Indels: 3
0.91 0.06 0.03
Matches are distributed among these distances:
31 13 0.14
32 48 0.53
33 30 0.33
ACGTcount: A:0.24, C:0.24, G:0.02, T:0.50
Consensus pattern (32 bp):
TTAAACTAACCTCTTCTTCTTTTACTATTTAG
Found at i:11813 original size:65 final size:64
Alignment explanation
Indices: 11737--11869 Score: 239
Period size: 65 Copynumber: 2.1 Consensus size: 64
11727 TTACCCAAAA
11737 TTAAACCAACCTCTTCTTCTTTTACTCTTTAGTTAAACTAACCTCTTCTTCTTTTACTATTTAAT
1 TTAAACCAACCTCTTCTTCTTTTACTCTTTAGTTAAACTAACCTCTTCTTC-TTTACTATTTAAT
* *
11802 TTAAACTAACCTCTTCTTCTTTTACTCTTTAGTTAAACTAACCTCTTCTTCTTTACTATTTAGT
1 TTAAACCAACCTCTTCTTCTTTTACTCTTTAGTTAAACTAACCTCTTCTTCTTTACTATTTAAT
11866 TTAA
1 TTAA
11870 TTACCTAGAA
Statistics
Matches: 66, Mismatches: 2, Indels: 1
0.96 0.03 0.01
Matches are distributed among these distances:
64 16 0.24
65 50 0.76
ACGTcount: A:0.25, C:0.23, G:0.02, T:0.50
Consensus pattern (64 bp):
TTAAACCAACCTCTTCTTCTTTTACTCTTTAGTTAAACTAACCTCTTCTTCTTTACTATTTAAT
Found at i:12858 original size:24 final size:23
Alignment explanation
Indices: 12831--12886 Score: 58
Period size: 24 Copynumber: 2.3 Consensus size: 23
12821 AAACTAAATT
* *
12831 AAGCCCAAATGCAAGGGAAAATCA
1 AAGCCCAAATG-AAAGGAAAATAA
* *
12855 AAGCCCCGAATGAAAGGAAATTAA
1 AAG-CCCAAATGAAAGGAAAATAA
12879 AAGCCCAA
1 AAGCCCAA
12887 CTATGCAAAT
Statistics
Matches: 26, Mismatches: 5, Indels: 3
0.76 0.15 0.09
Matches are distributed among these distances:
23 4 0.15
24 15 0.58
25 7 0.27
ACGTcount: A:0.50, C:0.21, G:0.20, T:0.09
Consensus pattern (23 bp):
AAGCCCAAATGAAAGGAAAATAA
Found at i:15519 original size:27 final size:27
Alignment explanation
Indices: 15462--15519 Score: 64
Period size: 27 Copynumber: 2.1 Consensus size: 27
15452 ATTCAAGGCT
* * *
15462 AAAAGGTCCAAATGCAAGTCAAAAGCT
1 AAAACGTCCAAATGCAAGCCAAAAGCC
*
15489 AAAACGTCCAAGTGCAAGACCAAAA-CC
1 AAAACGTCCAAATGCAAG-CCAAAAGCC
15516 AAAA
1 AAAA
15520 TGGTAACAAC
Statistics
Matches: 26, Mismatches: 4, Indels: 2
0.81 0.12 0.06
Matches are distributed among these distances:
27 21 0.81
28 5 0.19
ACGTcount: A:0.52, C:0.22, G:0.16, T:0.10
Consensus pattern (27 bp):
AAAACGTCCAAATGCAAGCCAAAAGCC
Found at i:17825 original size:27 final size:27
Alignment explanation
Indices: 17790--17841 Score: 77
Period size: 27 Copynumber: 1.9 Consensus size: 27
17780 ATCCTAGTCA
17790 AAGGCTACTGCCATATCAGATAAATGC
1 AAGGCTACTGCCATATCAGATAAATGC
* * *
17817 AAGGCTACTGCTATTTTAGATAAAT
1 AAGGCTACTGCCATATCAGATAAAT
17842 ATTGTCGTTG
Statistics
Matches: 22, Mismatches: 3, Indels: 0
0.88 0.12 0.00
Matches are distributed among these distances:
27 22 1.00
ACGTcount: A:0.37, C:0.17, G:0.17, T:0.29
Consensus pattern (27 bp):
AAGGCTACTGCCATATCAGATAAATGC
Found at i:18373 original size:32 final size:33
Alignment explanation
Indices: 18337--18417 Score: 89
Period size: 31 Copynumber: 2.6 Consensus size: 33
18327 TAATTTGGTT
*
18337 AATTAAGTCCCTAACGTTAG-AAAATCGGTTCA
1 AATTAAGTCCCTAACGTTAGCAAAATCGGCTCA
**
18369 AATT-AGTCCCTAACAAT-GCAAAATCGGCTCA
1 AATTAAGTCCCTAACGTTAGCAAAATCGGCTCA
* *
18400 AA-TAAGTTCCTAAAGTTA
1 AATTAAGTCCCTAACGTTA
18418 ACATTTTGAG
Statistics
Matches: 39, Mismatches: 7, Indels: 6
0.75 0.13 0.12
Matches are distributed among these distances:
30 2 0.05
31 33 0.85
32 4 0.10
ACGTcount: A:0.40, C:0.20, G:0.14, T:0.27
Consensus pattern (33 bp):
AATTAAGTCCCTAACGTTAGCAAAATCGGCTCA
Found at i:19271 original size:15 final size:16
Alignment explanation
Indices: 19237--19275 Score: 53
Period size: 15 Copynumber: 2.5 Consensus size: 16
19227 AAAAATTAAA
*
19237 TATTTTTATTTTAATG
1 TATTTTTATTTAAATG
*
19253 TATTTTTA-TTAAATT
1 TATTTTTATTTAAATG
19268 TATTTTTA
1 TATTTTTA
19276 ATAATTAAAA
Statistics
Matches: 21, Mismatches: 2, Indels: 1
0.88 0.08 0.04
Matches are distributed among these distances:
15 13 0.62
16 8 0.38
ACGTcount: A:0.28, C:0.00, G:0.03, T:0.69
Consensus pattern (16 bp):
TATTTTTATTTAAATG
Done.